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By I. Silver (Eds.)

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And Lovelock, J. , Studies in Air Hygiene, Medical Research Council Special Report Series N o . 262, London. 20. Hoorn, B. and Tyrrell, D. A. J. (1965). On the growth of certain "newer' respiratory viruses in organ culture. Brit. J. , 46, 109. 2 1 . Tyrrell, D. A. J. and Bynoe, M. L. (1965). Cultivation of a common cold virus in organ culture. Brit. Med. , i, 76. 22. Webster, L. T. (1933). Summarized by Wilson, G. S. and Miles, A. A. , Baltimore. 23. , Hill, A. , Topley, W. W. C. and Wilson, J.

Webster, L. T. (1933). Summarized by Wilson, G. S. and Miles, A. A. , Baltimore. 23. , Hill, A. , Topley, W. W. C. and Wilson, J. (1936). Experimental Epidemiology, Special Report Series N o . 209, Medical Research Council, London. TABLE Preparations tested for storage 1 of micro-organisms at reduced temperatures Agar 0-2% Ascorbic acid 001% Casein, isoelectric 2% Casein, purified 27c Casein, technical 27c Caseinate, sodium 27c a Gelatin 0 - 5 % , 2 % , 57c a Gelatin (27c) + skim milk ( 5 % ) Gelatin phosphate diluent a a 10%, 2 0 % , 5 0 % Horse serum Horse serum ( 2 5 % ) + s k i m milk (57c) Lactalbumin 27c Lactalbumin hydrolysate 27c Nutrient broth 1-5%, 37c Parenamine a Peptonized milk 3% Proteose peptone 0-2% Saline Skim milk 0 - 5 % , 5%, 10% Skim milk (5%)+gelatin ( 2 % ) + s t a r c h (0 0 5 % , 0 1 % , 0-5%, 1%) Skim milk (5%)+gelatin ( 2 % ) + h o r s e serum ( 1 0 % ) + s t a r c h (0 0 5 % , 0 1 % , 0-5%, 1%) Stuart's amino acid 1-5%, 3 % Stuart's medium Thioglycollate a Preparations giving 90 to 100% recovery after 6 weeks at — 7 0 ° C .

22 Proceedings Third International Symposium on Aerobiology 2. A subjective impression or history of recent or substantial U R I difficulties. 3. Bunk location on patrol: crew members occupying one group of 17 bunks had significantly more U R I , not alone in Patrols V through VIII, but also in two other submarines studied in Patrols II and XI. The reason for this difference is currently not known. Another unexpected association with increased U R I was that of C P O rating or of age over 35 years.

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